compounds of the microsource discovery spectrum (mssp) library Search Results


compounds of the microsource discovery spectrum (mssp) library  (MicroSource Discovery Systems)
90
MicroSource Discovery Systems compounds of the microsource discovery spectrum (mssp) library
Compounds Of The Microsource Discovery Spectrum (Mssp) Library, supplied by MicroSource Discovery Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/compounds of the microsource discovery spectrum (mssp) library/product/MicroSource Discovery Systems
Average 90 stars, based on 1 article reviews
compounds of the microsource discovery spectrum (mssp) library - by Bioz Stars, 2026-04
90/100 stars
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mssp library  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare mssp library
Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the <t>MSSP</t> <t>Library</t> and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.
Mssp Library, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mssp library/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
mssp library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
mssp library  (MicroSource Discovery Systems)
90
MicroSource Discovery Systems mssp library
Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the <t>MSSP</t> <t>Library</t> and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.
Mssp Library, supplied by MicroSource Discovery Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mssp library/product/MicroSource Discovery Systems
Average 90 stars, based on 1 article reviews
mssp library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the MSSP Library and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.

Journal: British Journal of Pharmacology

Article Title: Identification through high-throughput screening of 4'-methoxyflavone and 3',4'-dimethoxyflavone as novel neuroprotective inhibitors of parthanatos

doi: 10.1111/bph.12201

Figure Lengend Snippet: Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the MSSP Library and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.

Article Snippet: MSSP Library (2 mM) was purchased from the Johns Hopkins ChemCORE Facility (10 mM stock was originally purchased from MicroSource Discovery Inc., Groton, CT, USA).

Techniques: High Throughput Screening Assay, Fluorescence, Biomarker Discovery, Blocking Assay, Polymer, Cell Culture

Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the MSSP Library and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.

Journal: British Journal of Pharmacology

Article Title: Identification through high-throughput screening of 4'-methoxyflavone and 3',4'-dimethoxyflavone as novel neuroprotective inhibitors of parthanatos

doi: 10.1111/bph.12201

Figure Lengend Snippet: Flow chart for the screening project. Assay was developed and optimized in HeLa cells and then employed for high-throughput screen of two chemical libraries: the MSSP Library and the JHDL. AB (fluorescence-based) or CTG (luminescence-based) was used to quantify cell viability. Compounds showing toxicity were rescreened at 2 and 5 μM while those with marginal protective effects were rescreened at 20 μM. The 10 hits from the primary screen were rescreened for validation. One compound was confirmed as true hit. A number of small molecules belonging to the same class as this compound or similar classes were then selected and screened, resulting in one more hit that was then validated. Dose–response data were obtained for the two hits; they were proven to block the synthesis and accumulation of toxic PAR polymer and were also neuroprotective in cell culture. They are now to be investigated for their ability to afford neuroprotection in animal models of neuronal death.

Article Snippet: MSSP Library (2 mM) was purchased from the Johns Hopkins ChemCORE Facility (10 mM stock was originally purchased from MicroSource Discovery Inc., Groton, CT, USA).

Techniques: High Throughput Screening Assay, Fluorescence, Biomarker Discovery, Blocking Assay, Polymer, Cell Culture